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KMID : 0616120180490020145
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Korean Journal of Pharmacognosy
2018 Volume.49 No. 2 p.145 ~ p.154
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Molecular Identification and Chemical Analysis of Aconiti Kusnezoffii Tuber on the Domestic Markets
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Jang Hye-Ri
Joe Kyeong-Hwa
Song Kwang-Ho
Lee Kyoung-Jin
Park Sait-Byul
Lee Chan-Min
Ha In-Jin
Lee Kyung-Jin
Seh Young-Bae
Kim Yeong-Shik
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Abstract
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Aconiti Kusnezoffii Tuber has been traditionally used to treat the symptoms of rheumatoid arthritis and joint pain. The main constituents are diterpenoid alkaloids such as benzoylmesaconine, benzoylaconine, mesaconitine, aconitine, and hypaconitine. In Korea, Aconiti Kusnezoffii Tuber is officially defined as the tubers of Aconitum kusnezoffii Reichb., A. ciliare Decasisne, and A. triphyllum Nakai. On the other hand, only the tuber of A. kusnezoffii is to be used in China. In order to identify the botanical origin of Aconiti Kusnezoffii Tuber circulated in Korea, we analyzed 24 samples of Aconiti Kusnezoffii Tuber obtained from local markets for comparative DNA analysis. The sequence analysis of nrRNA ITS 1 was useful to distinguish Aconitum species and revealed that the roots of A. karakolicum were circulated in Korean markets without discretion. HPLC quantitative analysis showed that aconitine was detected at the highest amount in A. karakolicum. Authentic diterpenoid alkaloids were coinjected for quantification of aconitine-type ingredients. All data were statistically grouped by Principal Component Analysis (PCA). This study suggests that both molecular and chemical analyses should be utilized for the standardization and the quality control for Aconiti Kusnezoffii Tuber.
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KEYWORD
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Aconiti Kusnezoffii Tuber, DNA analysis, nrDNA ITS 1, PCA, Quantitative HPLC analysis
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